Product Name	Rabbit Anti-PLK4 Polyclonal Antibody
Cat No.	SLA0315
Chinese Name	丝氨酸/苏氨酸蛋白激酶18抗体
Alias	MCCRP2; SAK; STK18; 1700028H20; PLK4_HUMAN; PLK4; Polo-like kinase 4 (PLK-4); Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak; 2.7.11.21; PLK4_MOUSE; PLK4_RAT; polo like kinase 4; serine/threonine kinase 18; polo-like kinase 4 (Drosophila); polo-like kinase 4
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	WB (1:500-2000) Flow-Cyt (2ug/Test) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human PLK4/STK18
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	108 kDa
Observed MW.	110 kDa
Subcellular Location	Nucleus
Gene ID	10733
Swiss-Prot	O00444
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-STK18 (SLA0315) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kDa Observed band size: 110 kDa Figure 2. Blank control (black line) :MCF7. Primary Antibody (green line): Rabbit Anti-STK18 antibody (SLA0315) Dilution:2ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control (orange line) : Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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