Product Name	Rabbit Anti-DGAT2 Polyclonal Antibody
Cat No.	SLA0042
Chinese Name	甘油二酯酰基转移酶2抗体
Alias	ARAT; GS1999FULL; HMFN1045; 0610010B06Rik; DGAT-2; DGAT2_BOVIN; DGAT2; Acyl-CoA retinol O-fatty-acyltransferase (ARAT | Retinol O-fatty-acyltransferase); Diglyceride acyltransferase 2; 2.3.1.20; DGAT2_HUMAN; DGAT2_MOUSE; DGAT2_RAT; diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2 (mouse)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human DGAT2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	44 kDa
Observed MW.	44 kDa
Subcellular Location	Cytoplasm
Gene ID	84649
Swiss-Prot	Q96PD7
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. Acyl-coenzyme A:diacylglycerol acyltransferase, also known as DGAT1 and ARGP1, is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT1 plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism, such as intestinal fat absorption, lipoprotein assembly, adipose tissue form-ation and lactation. DGAT2, which has no homology to DGAT1, differs from DGAT1 in that its activity has been shown to be inhibited by MgCl in an in vitro assay. DGAT2 is expressed primarily in liver and white adipose tissue, which suggests that it plays an important role in mammalian triglyceride metabolism.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Rat Liver tissue lysates Primary: Anti-DGAT2 (SLA0042) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (DGAT2) Polyclonal Antibody, Unconjugated (SLA0042) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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