Product Name	Recombinant Rabbit Anti-SOX10 Monoclonal Antibody
Cat No.	SLA0826M
Chinese Name	转录因子SOX10重组兔单抗
Alias	DOM; PCWH; SOX-10; WS2E; WS4; WS4C; Sox21; gt; SOX10_HUMAN; SOX10; SOX10_MOUSE; Protein SOX-21; Transcription factor SOX-M; SOX10_RAT; SRY-box transcription factor 10; SRY (sex determining region Y)-box 10; SRY-box 10; dominant megacolon, mouse, human homolog of
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	26D7
React Species	Human,Mouse
Applications	WB (1:1000-5000) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human SOX10
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	50 kDa
Observed MW.	60 kDa
Subcellular Location	Nucleus
Gene ID	6663
Swiss-Prot	P56693
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SOX10 monoclonal antibody, unconjugated (SLA0826M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Figure 2. 4% Paraformaldehyde-fixed SK-MEL-28 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SOX10) monoclonal Antibody, unconjugated (SLA0826M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.