Product Name	Rabbit Anti-HIF1A Polyclonal Antibody
Cat No.	SLA0194
Chinese Name	缺氧诱导因子1α /低氧诱导因子-1抗体
Alias	HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; HIF1alpha; HIF1A_HUMAN; HIF1A; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS protein 1; PAS domain-containing protein 8; HIF1A_MOUSE; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Class E basic helix-loop-helix protein 78
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human HIF-1 Alpha protein
Purification	affinity purified by Protein A
Concentration	1mg, ml
Predicted MW.	92 kDa
Subcellular Location	Nucleus
Gene ID	3091
Swiss-Prot	Q16665
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Hypoxia-inducible factor 1 alpha is not only of particular significance for the body to maintain normal physiological functions under hypoxic conditions,and plays an important role in pathological processes such as tumor growth and neuronal apoptosis. HIF1 alpha can regulate the expression levels of many downstream genes. HIF appears in mammalian cells under hypoxic stress conditions. HIF is a transcription factor, stabilizes cells under hypoxia.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed Hela (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (SLA0194) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Figure 2. 4% Paraformaldehyde-fixed HepG2 (treated with 500uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (SLA0194) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.