Product Name	Rabbit Anti-Wnt11 Polyclonal Antibody
Cat No.	SLA0196
Chinese Name	信号通路Wnt11抗体
Alias	HWNT11; WNT11_HUMAN; WNT11; WNT11_MOUSE; Wnt-11; Wnt family member 11; wingless-type MMTV integration site family, member 11
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse
Applications	WB (1:500-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse Wnt11 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	39 kDa
Observed MW.	50 kDa
Subcellular Location	Extracellular
Gene ID	22411
Swiss-Prot	P48615
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene ”wingless” and to its vertebrate ortholog, Int-1, a mouse proto-oncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins which have features typical of secreted growth factors. Wnt-11 is expressed in the tips of ureteric buds and in the perichondrium, a stem cell-like layer that surrounds the future bones and directs their growth and regeneration. Wnt-11 activity is required for cells to undergo correct convergent extension movements during gastrulation. Human Wnt-11 is also expressed in the lung mesenchyme, the urorectal septum, the urogenital folds, the labioscrotal swellings, and the cortex of the adrenal gland. Unlike other Wnt family members, Wnt-11 is not expressed in the neuroepithelium of the central nervous system. Wnt-11, along with Wnt-8c, is ex-pressed in the posterior region of the chick embryo in the caudal paraxial mesoderm that underlies the prospective caudal neural plate. The gene which encodes Wnt-11 maps to human chromosome 11q13.5.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 20 ng rmWnt11-Trx-His protein (SL42347P) per lane probed with Wnt11 polyclonal antibody respectively, unconjugated (SLA0196) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with Wnt11 polyclonal antibody, unconjugated (SLA0196) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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• Price: USD, per unit (50ul / 100ul / 200ul)