Product Name	PHF2 | KDM7C Rabbit pAb
Cat No.	SL43571R
Chinese Name	PHF2蛋白抗体
Alias	CENP-35; GRC5; JHDM1E; KDM7C; PHF2_HUMAN; PHF2; PHD finger protein 2; 1.14.11.-; KIAA0662; PHF2_MOUSE; lysine demethylase 7C; jumonji C domain-containing histone demethylase 1E; centromere protein 35
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human PHF2 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	121 kDa
Observed MW.	34 kDa
Subcellular Location	Nucleus
Gene ID	5253
Swiss-Prot	O75151
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a protein which contains a zinc finger-like PHD (plant homeodomain) finger, distinct from other classes of zinc finger motifs, and a hydrophobic and highly conserved domain. The PHD finger shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human PHF2 protein, C-His(SL43571P) Primary: Anti-PHF2 (SL43571R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kDa Observed band size: 34 kDa

Figure 2. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (PHF2) Polyclonal Antibody, Unconjugated (SL43571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (PHF2) Polyclonal Antibody, Unconjugated (SL43571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.