ChaGo-K-1 Cell Specific Culture Medium - Basic Information

Culture Medium	88% RPMI-1640 + 10% FBS + 1% Glutamine + 1% Sodium Pyruvate
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, light yellow
Procedure	① Preparation: Expose the pipette, 50mL centrifuge tube, 100mL serum bottle, and laminar flow hood to UV light for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath; ② Preparation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically add 10% FBS, 1% glutamine, and 1% sodium pyruvate into the 88% RPMI-1640; ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and place the culture medium back into the 4°C refrigerator. Wipe the laminar flow hood with alcohol, extinguish the flame, and irradiate with UV light for 30 minutes.