IMR-90 Specialized Medium - Basic Information

Medium	88% EMEM + 10% FBS + 1% NEAA + 1% L-Glutamine.
Storage Conditions	Store at 2-8°C, valid for one month.
Appearance	Clear and transparent.
Procedure	① Preparation: Place the pipette, 50mL centrifuge tubes, 100mL serum bottles, and the laminar flow hood under UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath. ② Formulation: Flame-sterilize the openings of the basal medium bottle and serum bottle, then aseptically add 50mL of serum, 1% NEAA, and 1% L-Glutamine into 440mL of basal medium. ③ Sealing: Seal the culture medium bottle opening with sealing film, attach the corresponding product label, and return the culture medium to the 4°C refrigerator. Wipe the laminar flow hood with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.