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GT1-1 Cell-Specific Culture Medium - Basic Information
Culture Medium GT1-1 Cell-Specific Culture Medium: 90% DMEM/F-12 + 8% FBS + 2% HS Storage Conditions Store at 2-8°C, valid for one month Appearance Clear and transparent, pale yellow Procedure
① Preparation: Place pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the laminar flow hood under UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath;
② Preparation: Flame-sterilize the mouth of the basal medium bottle and serum bottle, then aseptically pour 40mL of FBS and 10mL of HS into 450mL of basal medium;
③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the culture medium to a 4°C refrigerator. Wipe down the laminar flow hood with alcohol, extinguish the flame, and UV-irradiate for 30 minutes;
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