HCT-8 Cell-Specific Culture Medium - Basic Information

Medium	HCT-8 Cell-Specific Culture Medium: 90% RPMI-1640 + 10% HS
Storage Condition	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, slightly yellowish
Procedure	① Preparation: UV-sterilize pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the clean bench for 20 minutes; pre-warm the basal medium in a constant temperature water bath at 37°C; ② Formulation: Sterilize the mouths of basal medium bottles and serum bottles with flame; aseptically pour 10% HS into 90% RPMI-1640; ③ Sealing: Seal the medium bottle mouth with sealing film, affix the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-sterilize for 30 minutes;