NK-92MI Cell-Specific Culture Medium - Basic Information

Culture Medium	NK-92MI Cell-Specific Culture Medium: MEMα + 0.2 mM Inositol + 0.1 mM β-mercaptoethanol + 0.02 mM Folic Acid + 12.5% HS + 12.5% FBS + 1% P/S
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, light red
Procedure	① Preparation: UV-irradiate pipettes, 50 mL centrifuge tubes, 100 mL serum bottles, and the clean bench for 20 minutes; pre-warm basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the basal medium bottle mouth and serum bottle mouth; aseptically pour the serum mixture (0.2 mM Inositol + 0.1 mM β-mercaptoethanol + 0.02 mM Folic Acid + 12.5% HS + 12.5% FBS + 1% P/S) into the basal medium. ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the culture medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.