Product Name	Rabbit Anti-JUN Polyclonal Antibody
Cat No.	SLA0150
Chinese Name	原癌基因蛋白/活化蛋白1抗体
Alias	AP-1; AP1; c-Jun; cJUN; p39; Junc; JUN_HUMAN; JUN; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; JUN_MOUSE; AH119; V-jun avian sarcoma virus 17 oncogene homolog (Jun A); JUN_PIG; JUN_RAT; Rjg-9; Jun proto-oncogene, AP-1 transcription factor subunit; jun oncogene
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	Flow-Cyt (1ug/Test) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human C-jun protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	36 kDa
Observed MW.	43/36 kDa
Subcellular Location	Nucleus
Gene ID	3725
Swiss-Prot	P05412
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	C-jun (Oncoprotein C-jun) is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Blank control (black line) :Hela. Primary Antibody (green line): Rabbit Anti-C-jun antibody (SLA0150) Dilution:1ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-BF488 Dilution: 0.5ug/Test. Isotype control (orange line) : Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

Figure 2. Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag (Cat. SL42049P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human C-jun Antibody, the EC50 is 180.9 ng/mL

Figure 3. Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag (Cat. SL42049P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human C-jun Antibody, the EC50 is 27.15 ng/mL

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.