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Product Name Rabbit Anti-ANXA10 Polyclonal Antibody Cat No. SLA0226 Chinese Name 膜粘连蛋白10抗体 Alias ANX14; RGD1560956; ANX10_HUMAN; ANXA10; Annexin-10; Annexin-14; ANX10_MOUSE; annexin A10 Host Species Rabbit Isotype IgG Clonality Polyclonal React Species Human,Mouse,Rat Applications WB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.Immunogen Recombinant human ANXA10 protein Purification affinity purified by Protein A Concentration 1mg/ml Predicted MW. 37 kDa Observed MW. 35-37 kDa Gene ID 11199 Swiss-Prot Q9UJ72 Storage 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Validation Images
Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ANXA10 polyclonal antibody, unconjugated (SLA0226) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ANXA10 Polyclonal Antibody, Unconjugated (SLA0226) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ANXA10 Polyclonal Antibody, Unconjugated (SLA0226) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ANXA10 Polyclonal Antibody, Unconjugated (SLA0226) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.
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