Product Name	Rabbit Anti-CD86 Polyclonal Antibody
Cat No.	SLA0242
Chinese Name	CD86抗体
Alias	B7-2; B7.2; B70; CD28LG2; LAB72; CD86_HUMAN; CD86; Activation B7-2 antigen; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86 molecule; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B-lymphocyte antigen B7-2
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD86 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	37 kDa
Observed MW.	68 kDa
Subcellular Location	Membrane
Gene ID	942
Swiss-Prot	P42081
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD86 polyclonal antibody, unconjugated (SLA0242) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. The MJ (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD86 antibody (SLA0242): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 3. 4% Paraformaldehyde-fixed Daudi (H) cell; Antibody incubation with (CD86) polyclonal Antibody, unconjugated (SLA0242) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.