Product Name	TNFRSF8 | CD30/Ki-1 Mouse mAb
Cat No.	SLM-43683M
Chinese Name	CD30单克隆抗体
Alias	TNR8_HUMAN; TNFRSF8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; CD30; D1S166E; TNF receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	2F2
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD30 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	62 kDa
Observed MW.	85-120 kDa
Subcellular Location	Cytoplasm
Gene ID	943
Swiss-Prot	P28908
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD30 monoclonal antibody, unconjugated (SLM-43683M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (SLM-43683M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (SLM-43683M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (SLM-43683M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.