Product Name	SERPINB3 | SCCA1/T4-A Recombinant Rabbit mAb
Cat No.	SLM-43190R
Chinese Name	丝氨酸蛋白酶抑制剂B3重组兔单抗
Alias	HsT1196; SCC; SCCA-1; SCCA-PD; SCCA1; SSCA1; T4-A; SPB3_HUMAN; SERPINB3; Protein T4-A; Squamous cell carcinoma antigen 1 (SCCA-1); SCCA; serpin family B member 3; squamous cell carcinoma antigen 1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	8C9
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human SERPINB3 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	45 kDa
Observed MW.	45 kDa
Subcellular Location	Cytoplasm
Gene ID	6317
Swiss-Prot	P29508
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase.SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SERPINB3 monoclonal antibody, unconjugated (SLM-43190R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (SLM-43190R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (SLM-43190R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (SLM-43190R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.