Product Name	PMEL | Melanoma gp100 Recombinant Rabbit mAb
Cat No.	SLM-61122R
Chinese Name	黑色素瘤相关抗原gp100重组兔单抗
Alias	D12S53E; HMB-45; HMB45; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV; gp100; D10H12S53E; D12S53Eh; gp87; PMEL_BOVIN; PMEL; Melanocyte protein Pmel 17; Premelanosome protein; Retinal pigment epithelial-specific protein; Silver locus protein homolog; RPE1; PMEL_HUMAN; ME20-M (ME20M); Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; PMEL_MOUSE; Silver locus protein; silver (mouse homolog) like; silver homolog (mouse)
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	11E7
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PMEL
Purification	affinity purified by Protein A
Predicted MW.	70 kDa
Observed MW.	100 kDa
Subcellular Location	Extracellular
Gene ID	6490
Swiss-Prot	P40967
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PMEL monoclonal antibody, unconjugated (SLM-61122R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PMEL Monoclonal Antibody, Unconjugated(SLM-61122R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. (Negative control)Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PMEL Monoclonal Antibody, Unconjugated(SLM-61122R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMEL | Melanoma gp100 Antibody, Unconjugated (SLM-61122R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.