Product Name	Recombinant Rabbit Anti-CST3 Monoclonal Antibody
Cat No.	SLA0423M
Chinese Name	胱抑素C/半胱氨酸蛋白酶抑制剂C单克隆抗体
Alias	ADLDWA; ARMD11; HEL-S-2; CysC; CYTC_HUMAN; CST3; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin; CYTC_MOUSE; CYTC_RAT; A0A8C0SEH9_CANLF; LOC607874; D5MTH1_FELCA;
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	7B2
React Species	Human
Applications	WB (1:500-1000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Cystatin C Protein
Purification	affinity purified by Protein G
Concentration	1mg/ml
Predicted MW.	14 kDa
Observed MW.	13 kDa
Subcellular Location	Secreted
Gene ID	1471
Swiss-Prot	P01034
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins(stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Cystatin C monoclonal antibody, unconjugated (SLA0423M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cystatin C) Monoclonal Antibody, Unconjugated (SLA0423M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cystatin C) Monoclonal Antibody, Unconjugated (ascites of SLA0423M 7B2) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Figure 4. Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cystatin C Monoclonal Antibody, Unconjugated (SLA0423M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.