Product Name	Rabbit Anti-RAD9A Polyclonal Antibody
Cat No.	SLA0305
Chinese Name	磷酸化细胞周期检查控制蛋白质抗体
Alias	RAD9; RAD9A_HUMAN; RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; 3.1.11.2; RAD9A_MOUSE; mRAD9; Rad9-like protein; RAD9 checkpoint clamp component A; RAD9 (S. pombe) homolog; RAD9 homolog A (S. pombe); RAD9A | RAD9 (phospho-S336); p-RAD9; phospho-RAD9; p-RAD9A; RAD9A | RAD9 (phospho-Ser336)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human RAD9 around the phosphorylation site of Ser336
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	43 kDa
Subcellular Location	Nucleus
Gene ID	5883
Swiss-Prot	Q99638
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded (Prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (SLA0305) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (SLA0305) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (SLA0305) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 4. Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (SLA0305) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.