Product Name	Recombinant Rabbit Anti-MUC1 Monoclonal Antibody
Cat No.	SLA0519M
Chinese Name	粘蛋白-1单克隆抗体
Alias	ADMCKD; ADMCKD1; ADTKD2; CA 15-3; CD227; Ca15-3; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM; MUC1_HUMAN; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3 (CA 15-3); Carcinoma-associated mucin; Episialin; Krebs von den Lungen-6 (KL-6); Peanut-reactive urinary mucin (PUM); Polymorphic epithelial mucin (PEM); Tumor-associated epithelial membrane antigen (EMA); Tumor-associated mucin; MUC1_MOUSE; mucin 1, cell surface associated; mucin 1, transmembrane; medullary cystic kidney disease 1 (autosomal dominant); epithelial membrane antigen
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	5B9
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant Human Mucin-1 Protein
Purification	affinity purified by Protein A
Predicted MW.	138 kDa
Subcellular Location	Nucleus
Gene ID	4582
Swiss-Prot	P15941
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (SLA0519M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (SLA0519M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mucin-1 Monoclonal Antibody, Unconjugated (SLA0519M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Figure 4. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mucin-1 Monoclonal Antibody, Unconjugated (SLA0519M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.