Product Name	Recombinant Rabbit Anti-TNFAIP3 Monoclonal Antibody
Cat No.	SLA1400M
Chinese Name	肿瘤坏死因子α诱导蛋白3重组兔单抗
Alias	TNAP3_HUMAN; TNFAIP3; TNF alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20; 2.3.2.-; 3.4.19.12; OTUD7C; TNAP3_MOUSE; Tnfip3; TNF alpha induced protein 3; tumor necrosis factor, alpha-induced protein 3
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	18F20
React Species	Human
Applications	IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Flow-Cyt (1:100-200) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human TNFAIP3
Purification	affinity purified by Protein A
Predicted MW.	90 kDa
Observed MW.	80 kDa
Subcellular Location	Nucleus
Gene ID	7128
Swiss-Prot	P21580
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	TNFAIP3 is cytokine-inducible protein that functions to inhibit apoptosis and activation of NF-κb. Play a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNFAIP3 Monoclonal Antibody, Unconjugated(SLA1400M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNFAIP3 Monoclonal Antibody, Unconjugated(SLA1400M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNFAIP3 Monoclonal Antibody, Unconjugated(SLA1400M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TNFAIP3 Monoclonal Antibody, Unconjugated(SLA1400M) at 1:100 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-TNFAIP3 antibody (SLA1400M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 6. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TNFAIP3) monoclonal Antibody, unconjugated (SLA1400M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.