Product Name	Recombinant Rabbit Anti-BAK1 Monoclonal Antibody
Cat No.	SLA1287M
Chinese Name	Bcl-2同源拮抗剂重组兔单抗
Alias	BAK; BAK-LIKE; BCL2L7; CDN1; N-BAK1; N-Bak; BAK_HUMAN; BAK1; Apoptosis regulator BAK; Bcl-2-like protein 7 (Bcl2-L-7); BAK_MOUSE; BCL2 antagonist/killer 1; BCL2-antagonist/killer 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5C2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:200-500) IHC-F (1:200-500) IF (1:200-500) Flow-Cyt (1ug/Test) ICC/IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human BAK1
Purification	affinity purified by Protein A
Predicted MW.	23 kDa
Observed MW.	25 kDa
Subcellular Location	Cytoplasm
Gene ID	578
Swiss-Prot	Q16611
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Bak monoclonal antibody, unconjugated (SLA1287M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated(SLA1287M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated(SLA1287M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated(SLA1287M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining
Figure 5. The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Bak antibody (SLA1287M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 6. 4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bak) monoclonal Antibody, unconjugated (SLA1287M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.