Product Name	Rabbit Anti-MYH1 Polyclonal Antibody
Cat No.	SLA0182
Chinese Name	心肌肌球蛋白重链抗体
Alias	HEL71; MYHSA1; MYHa; MyHC-2X/D; MyHC-2x; A530084A17Rik; IId; IId/x; MHC-2X/D; MHC2X/D; MYHC-IIX; MdMs; MyHC-IId/x; MyHC-IIx/d; Myhs-f; Myhs-f2; Myhsf2; MYHC; MYH1_BOVIN; MYH1; Myosin heavy chain 1; Myosin heavy chain 2x (MyHC-2x); Myosin heavy chain, skeletal muscle, adult 1; MYH1_HUMAN; Myosin heavy chain IIx/d (MyHC-IIx/d); MYH1_MOUSE; MYH1_PIG; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin heavy chain IIx/d
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human MYH1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	223 kDa
Observed MW.	44 kDa
Subcellular Location	Cytoplasm
Gene ID	4619
Swiss-Prot	P12882
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human MYH1 protein, N-His(SL42269P) Primary: Anti-MYH1 (SLA0182) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 44 kDa	Figure 2. Paraformaldehyde-fixed, paraffin embedded (mouse tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded (rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.	Figure 6. Negative sample: Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Figure 7. Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat liver);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.	Figure 8. Negative sample: Paraformaldehyde-fixed, paraffin embedded (human brain );Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Paraformaldehyde-fixed, paraffin embedded (human brain ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MYH1) Polyclonal Antibody, Unconjugated (SLA0182) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Mouse Tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH1 Polyclonal Antibody, Unconjugated (SLA0182) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.