DESCRIPTION

Murine Esm1 (Endothelial Specific Molecule-1/Endocan) is a proteoglycan secreted by vascular endothelial cells, with a core protein molecular weight of approximately 20 kDa. As a key regulator in vascular biology and the tumor microenvironment, its biological activity is highly dependent on a covalently linked dermatan sulfate glycosaminoglycan chain. Esm1 plays crucial roles in physiological angiogenesis and inflammatory responses by directly promoting endothelial cell proliferation and migration, as well as mediating leukocyte adhesion through interactions with integrins such as LFA-1. Under pathological conditions, it is significantly overexpressed in various malignant tumors and inflammatory diseases, driving abnormal neovascularization by enhancing signaling pathways like VEGF. Consequently, it has become an important biomarker for tumor angiogenesis and a potential indicator for inflammatory monitoring.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	932.3	51.5	5.5
2	8	236.3	18.0	7.6
3	8	121.2	8.6	7.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	918.1	41.0	4.5
2	16	237.6	15.4	6.5
3	16	124.1	9.5	7.7

RECOVERY

The recovery of mouse Esm1 spiked to three different levels throughout the range of the assay in mouse serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:40	88	78-98
	1:80	88	80-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:5.)

		Mouse serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	112
	Range (%)	109-115
1:8	Average% of Expected	112
	Range (%)	108-117
1:16	Average% of Expected	111
	Range (%)	105-118

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse Esm1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)