DESCRIPTION

Endoglin (CD105) is a homodimeric cell membrane glycoprotein that acts as an accessory receptor for transforming growth factor-beta (TGF-β) and is involved in vascular development and remodeling. It is highly expressed on vascular endothelial cells and plays a crucial role in angiogenesis, making it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. Endoglin is also involved in the regulation of vascular endothelial cell migration and has important implications for embryonic heart development. Additionally, it can regulate the response of endothelial cells to blood flow, influencing vascular remodeling and the establishment of normal vascular morphology during angiogenesis.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,064.7	37.8	1.8
2	8	523.0	6.9	1.3
3	8	148.2	4.3	2.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,032.2	54.1	2.7
2	16	511.8	13.6	2.7
3	16	148.5	5.2	3.5

RECOVERY

The recovery of mouse Endoglin/CD105 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
mouse serum	1:32	124	124-125
	1:64	108	87-120
Cell culture supernatant	1:4	91	88-94
	1:8	95	92-100
Tissue lysate	1:32	92	91-93
	1:64	93	89-95

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:8. The tissue lysate was initially diluted 1:16.)

		Mouse serum	Cell culture supernatant	Tissue lysate
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	111	97	104
	Range (%)	109-114	94-99	103-104
1:8	Average% of Expected	105	96	108
	Range (%)	101-110	96-97	107-109
1:16	Average% of Expected	109	94	116
	Range (%)	106-112	90-97	115-117

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse Endoglin/CD105 One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)