Product Name	Recombinant Rabbit Anti-SELPLG Monoclonal Antibody
Cat No.	SLA1858M
Chinese Name	P选择素糖蛋白配体1重组兔单抗
Alias	CD162; CLA; PSGL-1; PSGL1; Selpl; SELPL_HUMAN; SELPLG; Selectin P ligand; SELPL_MOUSE; Selp1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11D2
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PSGL 1
Purification	affinity purified by Protein A
Predicted MW.	43 kDa
Observed MW.	120 kDa
Subcellular Location	Membrane
Gene ID	6404
Swiss-Prot	Q14242
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The Human peripheral blood lymphocytes stained with SGL-1/CD162 for 30min at room temperature, followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PSGL-1/CD162 antibody (SLA1858M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Glioblastoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSGL-1/CD162 Monoclonal Antibody, Unconjugated(SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSGL-1/CD162 Monoclonal Antibody, Unconjugated (SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Pyogenic appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSGL-1/CD162 Monoclonal Antibody, Unconjugated (SLA1858M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SELPLG | PSGL-1/CD162 Monoclonal Antibody, Unconjugated (SLA1858M) at 1:2000 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining
Figure 11. The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PSGL-1/CD162 antibody (SLA1858M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 12. 4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (PSGL-1/CD162) monoclonal Antibody, unconjugated (SLA1858M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.