Product Name	Recombinant Rabbit Anti-FN1 Monoclonal Antibody
Cat No.	SLA2007M
Chinese Name	纤维连接蛋白重组兔单抗
Alias	FN; CIG; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF; E330027I09; Fn-1; FIBNEC; FINC_BOVIN; FN1; FINC_HUMAN; Cold-insoluble globulin (CIG); FINC_MOUSE; fibronectin 1; migration-stimulating factor; cold-insoluble globulin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11G3
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) ICC/IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Fibronectin
Purification	affinity purified by Protein A
Predicted MW.	272 kDa
Observed MW.	260 kDa
Subcellular Location	Extracellular
Gene ID	2335
Swiss-Prot	P02751
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Fibronectin monoclonal antibody, unconjugated (SLA2007M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FN1 | Fibronectin Monoclonal Antibody, Unconjugated (SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Fibronectin Monoclonal Antibody, Unconjugated(SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FN1 | Fibronectin Monoclonal Antibody, Unconjugated (SLA2007M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibronectin Monoclonal Antibody, Unconjugated (SLA2007M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibronectin Monoclonal Antibody, Unconjugated (SLA2007M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 13. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Fibronectin) monoclonal Antibody, unconjugated (SLA2007M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.