Product Name	Recombinant Rabbit Anti-CD8A Monoclonal Antibody
Cat No.	SLA0485M
Chinese Name	CD8A单克隆抗体
Alias	CD8; CD8alpha; IMD116; Leu2; p32; Ly-2; Ly-35; Ly-B; Lyt-2; CD8A_HUMAN; CD8A; T-lymphocyte differentiation antigen T8/Leu-2; MAL; CD8A_MOUSE; T-cell surface glycoprotein Lyt-2; Lyt2; G1SX34_RABIT; CD8 subunit alpha; CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain; CD8a molecule
Host Species	Mouse
Isotype	IgG1, k
Clonality	Monoclonal
Clone No.	4C8
React Species	Human
Applications	IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CD8A
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	27 kDa
Subcellular Location	Membrane
Gene ID	925
Swiss-Prot	P01732
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil. Merged staining of anti-CD8A (SLA0485M; 1:200; pink) anti-CD4 (SLM-52469R; 1:200; yellow) anti-CD3E (SLM-60002R; 1:200; light blue) anti-CD68 (SLM-60634R; 1:200; white) anti-CD11c (SLM-61135R; 1:200; orange) and anti-CD20 (SLA3106M; 1:200; green) DAPI (dark blue) was used as a nuclear counter stain.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human lymph nodes. Merged staining of anti-CD8A (SLA0485M; 1:200; pink) anti-CD4 (SLM-52469R; 1:200; yellow) and anti-CD3E (SLM-60002R; 1:200; light blue) DAPI (dark blue) was used as a nuclear counter stain.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:500 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, SL0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Figure 13. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8A Monoclonal Antibody, Unconjugated (SLA0485M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Rose pink, SL0296G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.