DESCRIPTION

Myocyte-specific enhancer factor 2D (MEF2D) is a transcription factor belonging to the MEF2 family, which plays critical roles in regulating gene expression during muscle development, neuronal differentiation, and immune cell function. MEF2D binds to specific DNA sequences in the promoter regions of target genes and interacts with various cofactors, including histone deacetylases and myogenic regulatory factors, to modulate transcriptional activity. Dysregulation of MEF2D has been implicated in several pathological conditions, including cancer, where it functions as either an oncogene or tumor suppressor depending on cellular context. In B-cell acute lymphoblastic leukemia (B-ALL), chromosomal translocations involving MEF2D lead to fusion proteins that drive leukemogenesis. Additionally, MEF2D contributes to neurodegenerative diseases through its role in neuronal survival and apoptosis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	4.87	0.26	5.34
2	8	1.44	0.07	4.86
3	8	0.77	0.04	5.19

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	4.92	0.23	4.67
2	16	1.41	0.10	7.09
3	16	0.81	0.05	6.17

RECOVERY

The recovery of human MEF2D spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:160	98	94-101

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:10.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	109
	Range (%)	108-110
1:8	Average% of Expected	114
	Range (%)	113-116
1:16	Average% of Expected	119
	Range (%)	116-122

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human MEF2D Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)