DESCRIPTION

CCL11 also named Eotaxin, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils, basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	232.3	1.9	0.8
2	8	57.0	3.0	5.3
3	8	29.4	0.9	3.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	236.4	5.9	2.5
2	16	57.6	3.8	6.6
3	16	30.1	0.9	3.0

RECOVERY

The recovery of mouse/rat CCL11/Eotaxin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:32	124	122-128
	1:64	123	120-126
Rat serum	1:80	124	120-127

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:4. The rat serum was initially diluted 1:10.)

		Mouse serum	Rat serum
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	115	98
	Range (%)	112-117	95-102
1:8	Average% of Expected	106	103
	Range (%)	100-111	100-106
1:16	Average% of Expected	109	103
	Range (%)	104-114	101-106

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse/Rat CCL11/Eotaxin One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)