DESCRIPTION

The transmembrane surface glycoprotein CD6 is a receptor expressed on thymocytes, mature T cells, the B1a B cell subset, and certain regions of the brain. It is a marker of T cells and an important T-cell regulator. CD6 acts as a costimulatory molecule synergizing with TCR or CD28 to enhance T cell proliferation. CD6 variants have been identified as susceptibility or disease modifier markers for multiple sclerosis, inflammatory bowel disease, Behçe's disease, psoriasis, and rheumatoid arthritis. Besides, Mouse soluble CD6 may have antibacterial effects, which can be used as adjunctive therapy in experimental models of complex and severe polymicrobial sepsis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,316.7	135.6	5.9
2	8	445.0	27.1	6.1
3	8	211.6	15.6	7.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,403.4	179.5	7.5
2	16	469.6	44.6	9.5
3	16	221.1	22.2	10.1

RECOVERY

The recovery of mouse CD6 spiked to three different levels throughout the range of the assay in mouse serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:16	99	95-106

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Mouse serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	105
	Range (%)	102-107
1:8	Average% of Expected	118
	Range (%)	116-119
1:16	Average% of Expected	130
	Range (%)	129-131

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD6 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)