DESCRIPTION

CD68 is a 110 kDa transmembrane glycoprotein widely expressed in monocyte cell types, such as macrophages, microglia, and osteoclasts. It is located at the lysosome and plasma membrane and is essential in various physiological and pathological processes, including atherosclerosis formation, inflammation and auto-immunity, bone-resorbing promotion, and tumor progression. It participates in the cellular response to lipopolysaccharide/oxidized low-density lipoprotein particle stimulus and negative regulation of dendritic cell antigen processing and presentation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	263.1	8.9	3.4
2	20	63.0	3.5	5.6
3	20	15.9	1.2	7.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	266.1	21.9	8.2
2	24	61.9	5.2	8.4
3	24	13.7	0.7	5.3

RECOVERY

The recovery of mouse CD68 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell culture supernatant	1:16	92	82-101
	1:32	87	78-94
Cell lysate	1:3,200	99	92-113
	1:6,400	100	93-112

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell culture supernatant was initially diluted 1:4. The cell lysate was initially diluted 1:400.)

		Cell culture supernatant	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	98	103
	Range (%)	94-101	103-104
1:8	Average% of Expected	102	101
	Range (%)	97-108	98-103
1:16	Average% of Expected	105	103
	Range (%)	95-119	102-103

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD68 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)