DESCRIPTION

CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	41.4	2.4	5.8
2	20	162.0	5.2	3.2
3	20	523.0	49.2	9.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	55.8	5.3	9.5
2	24	259.1	23.8	9.2
3	24	905.6	58.1	6.4

RECOVERY

The recovery of mouse CCL5 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:4	86	74-124
	1:8	87	75-115
Cell culture supernatant	1:2	107	83-119
	1:4	105	87-121

LINEARITY

To assess the linearity of the assay, mouse serum and cell culture supernatant samples were spiked with high concentrations of mouse CCL5 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (Mouse serum was initially diluted 1:4, cell culture supernatant was initially diluted 1:2.)

		Mouse serum(Sample Diluent PT 3-ec)	Cell culture supernatant(Sample Diluent PT 1-ef)
1:2	Average% of Expected	81	114
	Range (%)	74-86	105-126
1:4	Average% of Expected	88	108
	Range (%)	79-94	105-114
1:8	Average% of Expected	95	107
	Range (%)	86-103	93-119
1:16	Average% of Expected	97	98
	Range (%)	87-105	87-115

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CCL5 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)