DESCRIPTION

Thymus and Activation-Regulated Chemokine (TARC/CCL17) is a member of the CC or β-chemokine family. TARC/CCL17 is constitutively expressed in the thymus and is produced by dendritic cells (DC), endothelial cells, keratinocytes (KC) and fibroblasts. It is a ligand for CCR4, which is predominantly expressed on Th2 lymphocytes, basophils and natural killer cells. TARC has been shown to selectively recruit skin-homing CLA+ memory CD4+ T cells into skin during times of immune challenge. The serum TARC levels are closely related to the disease activity of Atopic dermatitis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	777.4	69.6	9.0
2	20	213.8	7.0	3.3
3	20	51.9	1.7	3.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	714.1	71.6	10.0
2	24	220.4	9.8	4.4
3	24	50.4	2.1	4.3

RECOVERY

The recovery of mouse CCL17/TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell culture supernatant	1:4	84	71-93
	1:8	98	76-129

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell culture supernatant
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	98
	Range (%)	84-117
1:8	Average% of Expected	104
	Range (%)	86-122
1:16	Average% of Expected	109
	Range (%)	105-113

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse CCL17/TARC Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)