DESCRIPTION

Uteroglobin, also named clara cell secretory protein or CC16, is mainly secreted by the club cells along the tracheobronchial tree, diffuses passively into the bloodstream and is rapidly eliminated by glomerular filtration. It is a steroid-dependent, immunomodulatory, cytokine-like protein, and it is secreted by the mucosal epithelial cells of many vertebrates and takes part in some signaling pathways. Growing evidence indicates that Uteroglobin has anti-inflammatory and anti-toxicant properties in the lung and may protect against obstructive lung diseases. Lower levels of Uteroglobin in blood and airways have been associated with prevalence and severity of Chronic obstructive pulmonary disease (COPD). Defects in Uteroglobin are associated with a susceptibility to asthma.

PRINCIPLE OF THE ASSAY

A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody also binds to the analyte. Horseradish peroxidase (HRP)-conjugated secondary antibody binds to the detection antibody. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	253.0	15.03	5.9
2	20	533.3	40.75	7.6
3	20	2,038.0	199.07	9.8

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	115.9	11.13	9.6
2	24	510.4	46.19	9.0
3	24	2,180.9	178.03	8.2

RECOVERY

The recovery of human Uteroglobin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:4	93	78-104
	1:8	107	97-121
Cell culture supernatant	1:2	96	80-111
	1:4	103	86-120
Urine	1:2	97	88-115
	1:4	100	83-119

LINEARITY

To assess the linearity of the assay, three samples were spiked with high concentrations of human Uteroglobin in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The plasma samples were initially diluted 1:2)

		Human plasma(Sample Diluent PT 3-df)	Cell culturesupernatant(Sample DiluentPT 1-df)	Urine(Sample Diluent PT 1-df)
1:2	Average% of Expected	90	122	110
	Range (%)	84-100	121-123	98-123
1:4	Average% of Expected	101	101	96
	Range (%)	87-115	92-107	88-107
1:8	Average% of Expected	108	92	95
	Range (%)	107-108	84-98	84-106
1:16	Average% of Expected	110	96	97
	Range (%)	102-118	93-103	86-109

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Uteroglobin produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)