DESCRIPTION

Lysosome-associated membrane glycoprotein 1 (LAMP1, also known as CD107a) is a glycosylated type I transmembrane protein that is crucial in lysosomal function and immune cell degranulation. It is enriched in the lysosomal membrane and provides selectins with carbohydrate ligands. LAMP1 is also a marker of degranulation in lymphocytes, such as CD8+ and NK cells, indicating its role in immune responses. Defects in LAMP1 can lead to impaired degranulation and reduced cytotoxicity, which are associated with conditions such as hemophagocytic lymphohistiocytosis. Additionally, LAMP1 is involved in tumor cell differentiation and metastasis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	625.6	41.3	6.6
2	8	151.2	6.7	4.4
3	8	74.4	3.2	4.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	644.0	37.3	5.8
2	16	158.8	15.2	9.6
3	16	77.4	6.5	8.4

RECOVERY

The recovery of human CD107a/LAMP1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:32	81	77-85
	1:64	80	71-85
Cell lysate	1:256	86	81-92
	1:512	98	88-108

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:4. The cell lysate was initially diluted 1:32.)

		Human serum	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	108	107
	Range (%)	108-109	106-108
1:8	Average% of Expected	119	119
	Range (%)	116-122	116-122
1:16	Average% of Expected	122	119
	Range (%)	119-124	116-121

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CD107a/LAMP1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)