DESCRIPTION

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily and is preferentially expressed on hematopoietic stem as well as progenitor cell subsets. It is a glycosylphosphatidylinositol (GPI)-anchored protein, first identified as a marker for activated T cells and platelets. CD109 stands out as the most potent negative regulator of canonical TGF-β signaling and responses. Additionally, CD109 might be a useful molecular target for the development of new therapeutics for malignant tumors, such as squamous cell carcinoma.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	22.17	1.27	5.75
2	8	5.56	0.14	2.45
3	8	1.95	0.03	1.73

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	22.55	1.37	6.09
2	16	5.55	0.20	3.53
3	16	1.88	0.11	5.72

RECOVERY

The recovery of human CD109 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:160	105	101-110
Cell lysate	1:32	86	70-97

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:10. The cell lysate was initially diluted 1:4.)

		Human plasma	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	99	90
	Range (%)	97-102	80-101
1:8	Average% of Expected	99	81
	Range (%)	98-100	75-87
1:16	Average% of Expected	102	92
	Range (%)	102-103	81-103

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD109 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)