DESCRIPTION

CD117, also known as c-Kit, is a type III receptor tyrosine kinase that plays a crucial role in cell signal transduction. It is activated by binding to its ligand, stem cell factor (SCF), leading to a phosphorylation cascade that activates various transcription factors in different cell types. This activation regulates apoptosis, cell differentiation, proliferation, chemotaxis, and cell adhesion. C-Kit is essential for the development and function of several cell types, including mast cells, hematopoietic stem cells, germ cells, melanocytes, and interstitial cells of Cajal in the gastrointestinal tract. It is also involved in processes such as hematopoiesis, gametogenesis, melanogenesis, and gastrointestinal motility.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	10.01	0.48	4.75
2	8	2.50	0.06	2.42
3	8	1.23	0.02	1.81

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	10.31	0.63	6.14
2	16	2.47	0.08	3.06
3	16	1.24	0.03	2.32

RECOVERY

The recovery of human CD117/c-Kit spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:400	106	99-110
	1:800	107	102-111
Cell culture supernatant	1:2	98	94-103
	1:4	101	97-109

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:50. )

		Human serum	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	104	96
	Range (%)	101-110	95-97
1:8	Average% of Expected	108	91
	Range (%)	105-115	82-99
1:16	Average% of Expected	109	-
	Range (%)	100-126	-

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CD117/c-Kit Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)