DESCRIPTION

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of interleukin 2.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,205.6	125.0	5.7
2	8	605.3	37.3	6.2
3	8	364.8	9.4	2.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,207.0	92.5	4.2
2	16	591.9	38.6	6.5
3	16	344.0	27.5	8.0

RECOVERY

The recovery of human CD122/IL-2RB spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:4	89	78-107
	1:8	81	73-86
Cell culture supernatant	1:2	100	98-102
	1:4	107	105-110
Cell lysate	1:128	100	97-102

LINEARITY

To assess the linearity of the assay, cell culture supernatant samples were spiked with high concentrations of human CD122/IL2RB and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Human plasma and cell lysate samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:8.)

		Human plasma	Cell culture supernatant	Cell lysate
1:2	Average% of Expected	100	91	100
	Range (%)	-	89-93	-
1:4	Average% of Expected	117	92	92
	Range (%)	113-121	90-93	87-96
1:8	Average% of Expected	104	94	92
	Range (%)	98-110	93-95	88-96
1:16	Average% of Expected	100	107	98
	Range (%)	95-105	103-112	93-103

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CD122/IL-2RB Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)