DESCRIPTION

UTX protein, also known as KDM6A, is a histone demethylase whose main function is to remove the methylation modification of lysine 27 (H3K27) on histone H3, thereby activating the transcription of target genes and playing a vital role in epigenetic regulation. UTX plays an important role in embryonic development and cell differentiation, affecting cell fate determination and tissue-specific function by regulating the expression of specific genes. This protein is a component of the COMPASS-like complex, which includes the histone methyltransferase MLL4 (KMT2D) and other related proteins.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	10.91	0.53	4.86
2	8	2.29	0.12	5.04
3	8	1.16	0.03	2.91

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	10.70	0.51	4.76
2	16	2.27	0.09	4.18
3	16	1.15	0.06	5.12

RECOVERY

The recovery of human UTX spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	117	110-126
	1:64	111	101-116

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	117
	Range (%)	107-128
1:8	Average% of Expected	107
	Range (%)	105-109
1:16	Average% of Expected	123
	Range (%)	118-128

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human UTX Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)