DESCRIPTION

Ubiquitin conjugation factor E4 B (UBE4B) is also named as Homozygously deleted in neuroblastoma 1 (HDNB1), KIAA0684 and UFD2. UBE4B is the mammalian homologue of Saccharomyces cerevisiae UFD2. As an important E3/E4 ubiquitin ligase, UBE4B is involved in biological processes such as embryonic development and neuronal protection and is associated with the development of various diseases. Indeed, functional abnormalities of UBE4B have been confirmed in several studies as a key regulatory factor of neurodegenerative diseases such as Alzheimer's disease (AD), Machado-Joseph disease (MJD), and peroneal muscular dystrophy (CMT). The role of UBE4B in tumors is dual, acting as both a tumor suppressor and a tumor promoter, depending on the tumor type and cellular environment.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,553.5	75.5	4.9
2	8	419.5	18.2	4.3
3	8	217.1	7.9	3.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,637.8	116.1	7.1
2	16	423.0	18.8	4.5
3	16	219.2	8.7	4.0

RECOVERY

The recovery of human UBE4B spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:40	82	81-84
	1:80	92	89-94

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:5.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	86
	Range (%)	81-92
1:8	Average% of Expected	82
	Range (%)	75-88
1:16	Average% of Expected	82
	Range (%)	78-87

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human UBE4B Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)