DESCRIPTION

TNFRSF13C, also known as BAFF receptor (BAFF-R) or CD268, is a receptor for B-cell activating factor (BAFF) and a member of the tumor necrosis factor receptor superfamily. This receptor is expressed mainly in B cells and is crucial for B cell survival and maturation. TNFRSF13C is the principal receptor required for BAFF-mediated mature B-cell survival and plays a significant role in regulating the peripheral B-cell population. Additionally, BAFF-R may be a novel treatment target for primary central nervous system lymphoma.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.38	0.15	2.79
2	8	1.39	0.03	1.97
3	8	0.73	0.02	2.94

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.52	0.24	4.29
2	16	1.33	0.07	5.11
3	16	0.74	0.03	3.45

RECOVERY

The recovery of human TNFRSF13C spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	90	86-93

LINEARITY

To assess the linearity of the assay, human serum samples and urine samples were spiked with high concentrations of human TNFRSF13C and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:2	Average% of Expected	78
	Range (%)	77-78
1:4	Average% of Expected	95
	Range (%)	94-95
1:8	Average% of Expected	106
	Range (%)	104-107
1:16	Average% of Expected	107
	Range (%)	104-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TNFRSF13C produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)