DESCRIPTION

Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B, also known as CD267) is a member of the tumor necrosis factor receptor superfamily. It is a receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS, binding both ligands with similar high affinity. TNFRSF13B plays a crucial role in humoral immunity by mediating activation of transcription factors and is involved in the stimulation of Band T-cell function. It also promotes T-cell independent antibody responses and plasma cell differentiation, counteracting BAFF-driven B-cell activation. Additionally, the concentration of plasma TNFSF13B may be correlated with immune thrombocytopenia.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	304.4	10.8	3.6
2	8	74.6	1.8	2.4
3	8	32.2	1.8	5.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	296.5	14.6	4.9
2	16	73.0	4.9	6.7
3	16	31.6	2.4	7.5

RECOVERY

The recovery of human TNFRSF13B/CD267 spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:4	79	75-82

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of human TNFRSF13B/CD267 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:2	Average% of Expected	96
	Range (%)	77-116
1:4	Average% of Expected	82
	Range (%)	80-84
1:8	Average% of Expected	108
	Range (%)	97-119
1:16	Average% of Expected	122
	Range (%)	122-123

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TNFRSF13B/CD267 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)