DESCRIPTION

TCEA1 (Transcription Elongation Factor A 1) is a key transcriptional regulatory protein belonging to the family of transcription elongation factors. It plays a significant role in eukaryotic cells by regulating gene expression through promoting the elongation of RNA polymerase II. TCEA1 can bind to the C-terminal domain (CTD) of RNA polymerase II, helping it transition from the promoter region to the elongation phase and maintaining transcriptional stability. TCEA1 is involved not only in the regulation of gene expression but also in DNA repair, cell cycle control, and cellular responses to environmental stimuli. In cancer research, the abnormal expression of TCEA1 is closely related to the occurrence and progression of tumors. For instance, in certain types of cancer, TCEA1 can activate the expression of oncogenes, promoting the proliferation and survival of tumor cells. Moreover, TCEA1 plays an important role in embryonic development and tissue differentiation, especially in erythrocyte differentiation and the transcriptional regulation of RNA polymerase II. The study of TCEA1 is of great significance for understanding the molecular mechanisms of gene expression and developing new cancer treatment strategies.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.37	0.08	1.57
2	8	1.42	0.05	3.68
3	8	0.75	0.03	4.45

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.40	0.21	3.82
2	16	1.40	0.05	3.27
3	16	0.75	0.03	4.40

RECOVERY

The recovery of human TCEA1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:64	113	109-116

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	108
	Range (%)	102-119
1:8	Average% of Expected	93
	Range (%)	86-107
1:16	Average% of Expected	92
	Range (%)	87-100

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TCEA1 Sandwich ELISA Kit produced at Sunlong Medical™.