DESCRIPTION

TCF7 (Transcription Factor 7), also known as TCF-1 or T-Cell Factor 1, is a member of the T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) family of high mobility group (HMG) box transcriptional activators. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	259.3	10.4	4.0
2	8	59.8	3.6	6.0
3	8	29.8	1.5	4.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	254.7	12.7	5.0
2	16	58.4	3.8	6.5
3	16	29.3	1.4	4.8

RECOVERY

The recovery of human TCF1/TCF7 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:64	106	102-114

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	84
	Range (%)	77-90
1:8	Average% of Expected	78
	Range (%)	74-83
1:16	Average% of Expected	94
	Range (%)	93-96

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TCF1/TCF7 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)