DESCRIPTION

TBX21, also known as T-bet, is a key T-box transcription factor that plays a critical role in the regulation of immune responses and the development of Th1 cells. The gene is a member of the phylogenetically conserved T-box family, which shares a common DNA-binding domain. T-bet is predominantly expressed in Th1 cells and natural killer (NK) cells, where it controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFN-γ). This transcription factor is essential for initiating Th1 lineage development from naïve T precursor cells.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,174.4	24.4	2.1
2	8	286.1	5.5	1.9
3	8	149.4	3.6	2.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,182.7	31.2	2.6
2	16	290.8	9.7	3.3
3	16	149.4	4.7	3.2

RECOVERY

The recovery of human TBX21/T-bet spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:640	83	70-94

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:40.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	102
	Range (%)	98-106
1:8	Average% of Expected	105
	Range (%)	96-114
1:16	Average% of Expected	97
	Range (%)	77-118

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TBX21/T-bet Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)