DESCRIPTION

REST (Repressor Element 1 Silencing Transcription Factor), also named as NRSF (neuron-restrictive silencer factor) or X2 box repressor, is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuronrestrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. It is also widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. Dysregulation of REST/NRSF has been implicated in various diseases, including nervous system disorders and cancers. In epilepsy, REST/NRSF has been shown to regulate the expression of potassium channels such as Kv7.2 and Kv7.3, influencing neuronal excitability and seizure activity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	516.2	29.3	5.7
2	8	121.5	6.5	5.4
3	8	36.3	1.2	3.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	553.0	45.3	8.2
2	16	128.4	9.2	7.2
3	16	36.9	1.1	3.1

RECOVERY

The recovery of human REST spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	119	115-127

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	82
	Range (%)	81-84
1:8	Average% of Expected	86
	Range (%)	85-88
1:16	Average% of Expected	87
	Range (%)	84-90

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human REST Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)