DESCRIPTION

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. RelB may exert an impact on Multiple sclerosis (MS) by modulating the functions of dendritic cells and the differentiation of T cells and B cells.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,470.8	54.7	3.7
2	8	393.9	19.9	5.0
3	8	194.1	4.0	2.0

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,556.7	103.6	6.7
2	16	418.5	33.5	8.0
3	16	202.6	19.5	9.6

RECOVERY

The recovery of human RELB spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:256	118	117-120
	1:512	116	115-120

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:32.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	111
	Range (%)	100-124
1:8	Average% of Expected	113
	Range (%)	94-129
1:16	Average% of Expected	114
	Range (%)	102-125

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human RELB Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)