Product Name	Recombinant Rabbit Anti-ADIPOQ Monoclonal Antibody
Cat No.	SLA1246M
Chinese Name	脂联素重组兔单抗
Alias	ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; 30kDa; APN; Ad; Adid; adipo; ADIPO_HUMAN; ADIPOQ; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein (ACRP30); Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein (apM-1); Gelatin-binding protein; ADIPO_MOUSE; Adipocyte-specific protein AdipoQ; adiponectin, C1Q and collagen domain containing; adipocyte, C1Q and collagen domain containing; adipose most abundant gene transcript 1; adiponectin precursor
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10B12
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Adiponectin
Purification	affinity purified by Protein A
Predicted MW.	26 kDa
Observed MW.	27 kDa
Gene ID	9370
Swiss-Prot	Q15848
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Adiponectin monoclonal antibody, unconjugated (SLA1246M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate tumors; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (SLA1246M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (SLA1246M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (SLA1246M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (SLA1246M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.