Product Name	Recombinant Rabbit Anti-HSPA12A Monoclonal Antibody
Cat No.	SLA1256M
Chinese Name	热休克蛋白70蛋白12A重组兔单抗
Alias	1700063D12Rik; D5Mgi40; Gm19925; mKIAA0417; HS12A_HUMAN; HSPA12A; Heat shock protein family A member 12A; KIAA0417; HS12A_MOUSE; heat shock protein family A (Hsp70) member 12A; heat shock 70kD protein 12A; heat shock 70kDa protein 12A
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7C4
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Hsp70 12A
Purification	affinity purified by Protein A
Predicted MW.	75 kDa
Observed MW.	75 kDa
Gene ID	259217
Swiss-Prot	O43301
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with HSPA12A monoclonal antibody, unconjugated (SLA1256M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (SLA1256M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (SLA1256M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (SLA1256M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (SLA1256M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.